EVALUATION OF AFLATOXIN B1 CONTENTS OF SELECTED FEED INGREDIENTS FROM FOUR MAJOR FEED MILLS IN IJEBU ODE AREA OF OGUN STATE
DOI :
https://doi.org/10.51791/njap.vi.4538Mots-clés :
Aflatoxin, Feed ingredients, Determination, Feed mill, ELISARésumé
This study was conducted to determine the level of aflatoxin B1 in four different poultry feed ingredients namely maize, wheat offal, soya bean meal and groundnut cake obtained from 4 selected feed mills ( A, B, C and D ) in Ijebu Ode, Ogun State of Nigeria. Each 0.5g sample of maize, wheat offal, soybean meal and groundnut cake were divided into four and ran separately with 5g of sodium chloride (Nacl) weighed and poured in a blender jar to determine the aflatoxin B1 level. Aflatoxin B1 level was determined with ELISA (Enzyme Linked Immunosorbent Assay) reader at 450nm. The results obtained were analysed
using statistical analysis software (SAS 1999) while the mean were compared using Duncan multiple range test. The analysed result of aflatoxin B1 levels in all the feed ingredients showed significant differences across all the feed mills. Aflatoxin B1 concentration in maize for feedmill A, B, C & D were 25.93, 13.10, 15.28 and 15.28ppb respectively. In soybean meal, aflatoxin B1 levels were 30.48, 24.78, 20.63 and 157.85ppb respectively for A, B, C & D respectively. Aflatoxin B1 concentration for
groundnut cake in A, B, C & D were 368.43, 289.28, 288.08 and 157.45ppb respectively. Wheat offal in A, B, C & D had mean values of 36.63, 49.84, 26.88 and 47.63ppb respectively. It was found from the study that in most cases, the aflatoxin B1 levels of all feed ingredients determined were above the 20ppb safe level for animal consumption. Therefore, feed ingredients in Ijebu Ode local government area during the period of the study were not safe for human or animal feed formulation.
